Cloned (Comment) | Organism |
---|---|
gene tryS, DNA and amino acid sequence determination and analysis, sequence comparisons, real-time PCR expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Leishmania donovani |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | subcellular localization study, overview | Leishmania donovani | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + glutathionylspermidine + ATP | Leishmania donovani | - |
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + glutathionylspermidine + ATP | Leishmania donovani MHOM / IN / 80 / Dd8 | - |
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + glutathionylspermidine + ATP | Leishmania donovani MHOM / IN / 83 / Ag | - |
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | Leishmania donovani | - |
glutathionylspermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | Leishmania donovani MHOM / IN / 80 / Dd8 | - |
glutathionylspermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | Leishmania donovani MHOM / IN / 83 / Ag | - |
glutathionylspermidine + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | Q8IFU8 | - |
- |
Leishmania donovani MHOM / IN / 80 / Dd8 | Q8IFU8 | - |
- |
Leishmania donovani MHOM / IN / 83 / Ag | Q8IFU8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by tag cleavage though thrombin, the tag is removed by benzamidine affinity chromatography, the purified enzyme is dialysed | Leishmania donovani |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | stage-dependent expression and up-regulation of trypanothione synthetase in amphotericin B resistant Leishmania donovani. LdTryS is constitutively transcribed in the exponential as well as the stationary phase, the latter shows higher enzyme expression | Leishmania donovani | - |
promastigote | LdTryS is overexpressed 2fold in Amp B resistant and stationary phase promastigotes compared to sensitive strain and logarithmic phase, respectively | Leishmania donovani | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + glutathionylspermidine + ATP | - |
Leishmania donovani | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + glutathionylspermidine + ATP | - |
Leishmania donovani MHOM / IN / 80 / Dd8 | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + glutathionylspermidine + ATP | - |
Leishmania donovani MHOM / IN / 83 / Ag | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | - |
Leishmania donovani | glutathionylspermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | - |
Leishmania donovani MHOM / IN / 80 / Dd8 | glutathionylspermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | - |
Leishmania donovani MHOM / IN / 83 / Ag | glutathionylspermidine + ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LdTryS | - |
Leishmania donovani |
TryS | - |
Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Leishmania donovani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | activity range, profile overview | Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Leishmania donovani |
Organism | Comment | Expression |
---|---|---|
Leishmania donovani | H2O2 treatment up to 0.150 mM for 8 hrs leads to 2fold increased expression of LdTryS probably to cope up with oxidative stress generated by H2O2. Stage-dependent up-regulation of trypanothione synthetase in amphotericin B resistant Leishmania donovani | up |
General Information | Comment | Organism |
---|---|---|
metabolism | trypanothione synthetase (TryS) of thiol metabolic pathway is the sole enzyme responsible for the biosynthesis of trypanothione in Leishmania donovani | Leishmania donovani |
physiological function | the enzyme is involved in Amp B resistance and during oxidative stress to help the parasites survival | Leishmania donovani |